Acute nutrient regulation of the mitochondrial glutathione redox state in pancreatic β-cells.
Identifieur interne : 000683 ( Main/Exploration ); précédent : 000682; suivant : 000684Acute nutrient regulation of the mitochondrial glutathione redox state in pancreatic β-cells.
Auteurs : Hilton K. Takahashi [Belgique] ; Laila R B. Santos [Belgique] ; Letícia P. Roma [Belgique] ; Jessica Duprez [Belgique] ; Christophe Broca [France] ; Anne Wojtusciszyn [France] ; Jean-Christophe Jonas [Belgique]Source :
- The Biochemical journal [ 1470-8728 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Calcium (métabolisme), Catalase (métabolisme), Cellules HEK293 (MeSH), Cellules à insuline (effets des médicaments et des substances chimiques), Cellules à insuline (métabolisme), Concentration en ions d'hydrogène (MeSH), Cytosol (métabolisme), Espèces réactives de l'oxygène (métabolisme), Glucose (pharmacologie), Glutathion (métabolisme), Humains (MeSH), Insuline (métabolisme), Mitochondries (effets des médicaments et des substances chimiques), Mitochondries (physiologie), Ménadione (métabolisme), NADP (métabolisme), Noyau de la cellule (métabolisme), Oxydoréduction (MeSH), Peroxyde d'hydrogène (métabolisme), Rats (MeSH), Sécrétion d'insuline (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Cellules à insuline, Mitochondries.
- métabolisme : Calcium, Catalase, Cellules à insuline, Cytosol, Espèces réactives de l'oxygène, Glutathion, Insuline, Ménadione, NADP, Noyau de la cellule, Peroxyde d'hydrogène.
- pharmacologie : Glucose.
- physiologie : Mitochondries.
- Animaux, Cellules HEK293, Concentration en ions d'hydrogène, Humains, Oxydoréduction, Rats, Sécrétion d'insuline.
English descriptors
- KwdEn :
- Animals (MeSH), Calcium (metabolism), Catalase (metabolism), Cell Nucleus (metabolism), Cytosol (metabolism), Glucose (pharmacology), Glutathione (metabolism), HEK293 Cells (MeSH), Humans (MeSH), Hydrogen Peroxide (metabolism), Hydrogen-Ion Concentration (MeSH), Insulin (metabolism), Insulin Secretion (MeSH), Insulin-Secreting Cells (drug effects), Insulin-Secreting Cells (metabolism), Mitochondria (drug effects), Mitochondria (physiology), NADP (metabolism), Oxidation-Reduction (MeSH), Rats (MeSH), Reactive Oxygen Species (metabolism), Vitamin K 3 (metabolism).
- MESH :
- chemical , metabolism : Calcium, Catalase, Glutathione, Hydrogen Peroxide, Insulin, NADP, Reactive Oxygen Species, Vitamin K 3.
- drug effects : Insulin-Secreting Cells, Mitochondria.
- metabolism : Cell Nucleus, Cytosol, Insulin-Secreting Cells.
- chemical , pharmacology : Glucose.
- physiology : Mitochondria.
- Animals, HEK293 Cells, Humans, Hydrogen-Ion Concentration, Insulin Secretion, Oxidation-Reduction, Rats.
Abstract
The glucose stimulation of insulin secretion by pancreatic β-cells depends on increased production of metabolic coupling factors, among which changes in NADPH and ROS (reactive oxygen species) may alter the glutathione redox state (EGSH) and signal through changes in thiol oxidation. However, whether nutrients affect EGSH in β-cell subcellular compartments is unknown. Using redox-sensitive GFP2 fused to glutaredoxin 1 and its mitochondria-targeted form, we studied the acute nutrient regulation of EGSH in the cytosol/nucleus or the mitochondrial matrix of rat islet cells. These probes were mainly expressed in β-cells and reacted to low concentrations of exogenous H2O2 and menadione. Under control conditions, cytosolic/nuclear EGSH was close to -300 mV and unaffected by glucose (from 0 to 30 mM). In comparison, mitochondrial EGSH was less negative and rapidly regulated by glucose and other nutrients, ranging from -280 mV in the absence of glucose to -299 mV in 30 mM glucose. These changes were largely independent from changes in intracellular Ca(2+) concentration and in mitochondrial pH. They were unaffected by overexpression of SOD2 (superoxide dismutase 2) and mitochondria-targeted catalase, but were inversely correlated with changes in NAD(P)H autofluorescence, suggesting that they indirectly resulted from increased NADPH availability rather than from changes in ROS concentration. Interestingly, the opposite regulation of mitochondrial EGSH and NAD(P)H autofluorescence by glucose was also observed in human islets isolated from two donors. In conclusion, the present study demonstrates that glucose and other nutrients acutely reduce mitochondrial, but not cytosolic/nuclear, EGSH in pancreatic β-cells under control conditions.
DOI: 10.1042/BJ20131361
PubMed: 24678915
Affiliations:
- Belgique, France
- Languedoc-Roussillon, Occitanie (région administrative), Province du Brabant wallon, Région de Bruxelles-Capitale, Région wallonne
- Bruxelles, Louvain-la-Neuve
- Université catholique de Louvain
Links toward previous steps (curation, corpus...)
Le document en format XML
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Takahashi</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Santos, Laila R B" sort="Santos, Laila R B" uniqKey="Santos L" first="Laila R B" last="Santos">Laila R B. 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Roma</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Duprez, Jessica" sort="Duprez, Jessica" uniqKey="Duprez J" first="Jessica" last="Duprez">Jessica Duprez</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Broca, Christophe" sort="Broca, Christophe" uniqKey="Broca C" first="Christophe" last="Broca">Christophe 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Takahashi</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Santos, Laila R B" sort="Santos, Laila R B" uniqKey="Santos L" first="Laila R B" last="Santos">Laila R B. Santos</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Roma, Leticia P" sort="Roma, Leticia P" uniqKey="Roma L" first="Letícia P" last="Roma">Letícia P. Roma</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Duprez, Jessica" sort="Duprez, Jessica" uniqKey="Duprez J" first="Jessica" last="Duprez">Jessica Duprez</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Broca, Christophe" sort="Broca, Christophe" uniqKey="Broca C" first="Christophe" last="Broca">Christophe Broca</name><affiliation wicri:level="3"><nlm:affiliation>†Laboratory for Diabetes Cell Therapy, Institute for Research in Biotherapy, University Hospital St-Eloi, Montpellier 34295, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>†Laboratory for Diabetes Cell Therapy, Institute for Research in Biotherapy, University Hospital St-Eloi, Montpellier 34295</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Wojtusciszyn, Anne" sort="Wojtusciszyn, Anne" uniqKey="Wojtusciszyn A" first="Anne" last="Wojtusciszyn">Anne Wojtusciszyn</name><affiliation wicri:level="3"><nlm:affiliation>†Laboratory for Diabetes Cell Therapy, Institute for Research in Biotherapy, University Hospital St-Eloi, Montpellier 34295, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>†Laboratory for Diabetes Cell Therapy, Institute for Research in Biotherapy, University Hospital St-Eloi, Montpellier 34295</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Jonas, Jean Christophe" sort="Jonas, Jean Christophe" uniqKey="Jonas J" first="Jean-Christophe" last="Jonas">Jean-Christophe Jonas</name><affiliation wicri:level="4"><nlm:affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author></analytic><series><title level="j">The Biochemical journal</title><idno type="eISSN">1470-8728</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Calcium (metabolism)</term><term>Catalase (metabolism)</term><term>Cell Nucleus (metabolism)</term><term>Cytosol (metabolism)</term><term>Glucose (pharmacology)</term><term>Glutathione (metabolism)</term><term>HEK293 Cells (MeSH)</term><term>Humans (MeSH)</term><term>Hydrogen Peroxide (metabolism)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Insulin (metabolism)</term><term>Insulin Secretion (MeSH)</term><term>Insulin-Secreting Cells (drug effects)</term><term>Insulin-Secreting Cells (metabolism)</term><term>Mitochondria (drug effects)</term><term>Mitochondria (physiology)</term><term>NADP (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Rats (MeSH)</term><term>Reactive Oxygen Species (metabolism)</term><term>Vitamin K 3 (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Calcium (métabolisme)</term><term>Catalase (métabolisme)</term><term>Cellules HEK293 (MeSH)</term><term>Cellules à insuline (effets des médicaments et des substances chimiques)</term><term>Cellules à insuline (métabolisme)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Cytosol (métabolisme)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Glucose (pharmacologie)</term><term>Glutathion (métabolisme)</term><term>Humains (MeSH)</term><term>Insuline (métabolisme)</term><term>Mitochondries (effets des médicaments et des substances chimiques)</term><term>Mitochondries (physiologie)</term><term>Ménadione (métabolisme)</term><term>NADP (métabolisme)</term><term>Noyau de la cellule (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Rats (MeSH)</term><term>Sécrétion d'insuline (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>Catalase</term><term>Glutathione</term><term>Hydrogen Peroxide</term><term>Insulin</term><term>NADP</term><term>Reactive Oxygen Species</term><term>Vitamin K 3</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Insulin-Secreting Cells</term><term>Mitochondria</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Cellules à insuline</term><term>Mitochondries</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>Cytosol</term><term>Insulin-Secreting Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calcium</term><term>Catalase</term><term>Cellules à insuline</term><term>Cytosol</term><term>Espèces réactives de l'oxygène</term><term>Glutathion</term><term>Insuline</term><term>Ménadione</term><term>NADP</term><term>Noyau de la cellule</term><term>Peroxyde d'hydrogène</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Glucose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Glucose</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mitochondries</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mitochondria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>HEK293 Cells</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Insulin Secretion</term><term>Oxidation-Reduction</term><term>Rats</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HEK293</term><term>Concentration en ions d'hydrogène</term><term>Humains</term><term>Oxydoréduction</term><term>Rats</term><term>Sécrétion d'insuline</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The glucose stimulation of insulin secretion by pancreatic β-cells depends on increased production of metabolic coupling factors, among which changes in NADPH and ROS (reactive oxygen species) may alter the glutathione redox state (EGSH) and signal through changes in thiol oxidation. However, whether nutrients affect EGSH in β-cell subcellular compartments is unknown. Using redox-sensitive GFP2 fused to glutaredoxin 1 and its mitochondria-targeted form, we studied the acute nutrient regulation of EGSH in the cytosol/nucleus or the mitochondrial matrix of rat islet cells. These probes were mainly expressed in β-cells and reacted to low concentrations of exogenous H2O2 and menadione. Under control conditions, cytosolic/nuclear EGSH was close to -300 mV and unaffected by glucose (from 0 to 30 mM). In comparison, mitochondrial EGSH was less negative and rapidly regulated by glucose and other nutrients, ranging from -280 mV in the absence of glucose to -299 mV in 30 mM glucose. These changes were largely independent from changes in intracellular Ca(2+) concentration and in mitochondrial pH. They were unaffected by overexpression of SOD2 (superoxide dismutase 2) and mitochondria-targeted catalase, but were inversely correlated with changes in NAD(P)H autofluorescence, suggesting that they indirectly resulted from increased NADPH availability rather than from changes in ROS concentration. Interestingly, the opposite regulation of mitochondrial EGSH and NAD(P)H autofluorescence by glucose was also observed in human islets isolated from two donors. In conclusion, the present study demonstrates that glucose and other nutrients acutely reduce mitochondrial, but not cytosolic/nuclear, EGSH in pancreatic β-cells under control conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24678915</PMID><DateCompleted><Year>2014</Year><Month>08</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1470-8728</ISSN><JournalIssue CitedMedium="Internet"><Volume>460</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Jun</Month><Day>15</Day></PubDate></JournalIssue><Title>The Biochemical journal</Title><ISOAbbreviation>Biochem J</ISOAbbreviation></Journal><ArticleTitle>Acute nutrient regulation of the mitochondrial glutathione redox state in pancreatic β-cells.</ArticleTitle><Pagination><MedlinePgn>411-23</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1042/BJ20131361</ELocationID><Abstract><AbstractText>The glucose stimulation of insulin secretion by pancreatic β-cells depends on increased production of metabolic coupling factors, among which changes in NADPH and ROS (reactive oxygen species) may alter the glutathione redox state (EGSH) and signal through changes in thiol oxidation. However, whether nutrients affect EGSH in β-cell subcellular compartments is unknown. Using redox-sensitive GFP2 fused to glutaredoxin 1 and its mitochondria-targeted form, we studied the acute nutrient regulation of EGSH in the cytosol/nucleus or the mitochondrial matrix of rat islet cells. These probes were mainly expressed in β-cells and reacted to low concentrations of exogenous H2O2 and menadione. Under control conditions, cytosolic/nuclear EGSH was close to -300 mV and unaffected by glucose (from 0 to 30 mM). In comparison, mitochondrial EGSH was less negative and rapidly regulated by glucose and other nutrients, ranging from -280 mV in the absence of glucose to -299 mV in 30 mM glucose. These changes were largely independent from changes in intracellular Ca(2+) concentration and in mitochondrial pH. They were unaffected by overexpression of SOD2 (superoxide dismutase 2) and mitochondria-targeted catalase, but were inversely correlated with changes in NAD(P)H autofluorescence, suggesting that they indirectly resulted from increased NADPH availability rather than from changes in ROS concentration. Interestingly, the opposite regulation of mitochondrial EGSH and NAD(P)H autofluorescence by glucose was also observed in human islets isolated from two donors. In conclusion, the present study demonstrates that glucose and other nutrients acutely reduce mitochondrial, but not cytosolic/nuclear, EGSH in pancreatic β-cells under control conditions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Takahashi</LastName><ForeName>Hilton K</ForeName><Initials>HK</Initials><AffiliationInfo><Affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santos</LastName><ForeName>Laila R B</ForeName><Initials>LR</Initials><AffiliationInfo><Affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roma</LastName><ForeName>Letícia P</ForeName><Initials>LP</Initials><AffiliationInfo><Affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duprez</LastName><ForeName>Jessica</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Broca</LastName><ForeName>Christophe</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>†Laboratory for Diabetes Cell Therapy, Institute for Research in Biotherapy, University Hospital St-Eloi, Montpellier 34295, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wojtusciszyn</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>†Laboratory for Diabetes Cell Therapy, Institute for Research in Biotherapy, University Hospital St-Eloi, Montpellier 34295, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jonas</LastName><ForeName>Jean-Christophe</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>*Université catholique de Louvain, Institut de recherche expérimentale et clinique, Pôle d'endocrinologie, diabète et nutrition, Brussels B-1200, Belgium.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biochem J</MedlineTA><NlmUniqueID>2984726R</NlmUniqueID><ISSNLinking>0264-6021</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007328">Insulin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>53-59-8</RegistryNumber><NameOfSubstance UI="D009249">NADP</NameOfSubstance></Chemical><Chemical><RegistryNumber>723JX6CXY5</RegistryNumber><NameOfSubstance UI="D024483">Vitamin K 3</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.6</RegistryNumber><NameOfSubstance UI="D002374">Catalase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance 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UI="D003600" MajorTopicYN="N">Cytosol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007328" MajorTopicYN="N">Insulin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000078790" MajorTopicYN="N">Insulin Secretion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050417" MajorTopicYN="N">Insulin-Secreting Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009249" MajorTopicYN="N">NADP</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024483" MajorTopicYN="N">Vitamin K 3</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>4</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>4</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>8</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24678915</ArticleId><ArticleId IdType="pii">BJ20131361</ArticleId><ArticleId IdType="doi">10.1042/BJ20131361</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li><li>Province du Brabant wallon</li><li>Région de Bruxelles-Capitale</li><li>Région wallonne</li></region><settlement><li>Bruxelles</li><li>Louvain-la-Neuve</li></settlement><orgName><li>Université catholique de Louvain</li></orgName></list><tree><country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Takahashi, Hilton K" sort="Takahashi, Hilton K" uniqKey="Takahashi H" first="Hilton K" last="Takahashi">Hilton K. Takahashi</name></region><name sortKey="Duprez, Jessica" sort="Duprez, Jessica" uniqKey="Duprez J" first="Jessica" last="Duprez">Jessica Duprez</name><name sortKey="Jonas, Jean Christophe" sort="Jonas, Jean Christophe" uniqKey="Jonas J" first="Jean-Christophe" last="Jonas">Jean-Christophe Jonas</name><name sortKey="Roma, Leticia P" sort="Roma, Leticia P" uniqKey="Roma L" first="Letícia P" last="Roma">Letícia P. Roma</name><name sortKey="Santos, Laila R B" sort="Santos, Laila R B" uniqKey="Santos L" first="Laila R B" last="Santos">Laila R B. Santos</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Broca, Christophe" sort="Broca, Christophe" uniqKey="Broca C" first="Christophe" last="Broca">Christophe Broca</name></region><name sortKey="Wojtusciszyn, Anne" sort="Wojtusciszyn, Anne" uniqKey="Wojtusciszyn A" first="Anne" last="Wojtusciszyn">Anne Wojtusciszyn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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